This past week we were able to start our experiment. Our measurements of our concentrations have been placed in 30 mL tubes. To first start our experiment we trypsinized our cells and placed them into a different tube for us to to be able to insert the new DMEM feeding medium. After our cells were mixed with the medium we separated them into the 7 different well plates with 2 mL in each. Unfortunately, when we went to feed our cells with our experimental media we noticed that our cells had been contaminated with mold. We had to scrap those well plates and start over.
When we started our experiment for the second time we used the FBS medium instead and made sure we ethanoled everything tons of times. When we checked these new cells under the microscope we noticed that we had a higher amount of cells than we did in our first trial. Since we had to start over we currently do not have any cell counts but we are working to start counting our new batch of cells.
Another task of this week was to edit our procedure again to make sure we had the right information and to insert the changes we had made while starting our experiment.
When we started our experiment for the second time we used the FBS medium instead and made sure we ethanoled everything tons of times. When we checked these new cells under the microscope we noticed that we had a higher amount of cells than we did in our first trial. Since we had to start over we currently do not have any cell counts but we are working to start counting our new batch of cells.
Another task of this week was to edit our procedure again to make sure we had the right information and to insert the changes we had made while starting our experiment.